pegfp n1 pd l1 (Addgene inc)
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pegfp n1 pd l1
Pegfp N1 Pd L1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+n1+pd+l1/pEGFP-N1%2FPD-L1+(Plasmid+%23121478)/pmc12971183-25-13-23
Average 93 stars, based on 8 article reviews
Pegfp N1 Pd L1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+n1+pd+l1/pEGFP-N1%2FPD-L1+(Plasmid+%23121478)/pmc12971183-25-13-23
Average 93 stars, based on 8 article reviews
pegfp n1 pd l1 - by Bioz Stars,
2026-09
93/100 stars
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Transfection:Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL −1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins. Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of PEI and plasmids including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins Article Snippet: HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL −1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including pEGFP-N1/PD-L1 (addgene Cat# 121478) and BFP-Rab5 (addgene Cat# 49147) in serum-free DMEM for 24 h. The transfected cells were further incubated with 0.5 μM CVR-EPLA for various times, fixed with a 4% PFA solution for 15 min, and finally imaged using a confocal laser scanning microscope (NIS Viewer 5.21 and NIS-Elements Viewer 5.21.00, Nikon, Japan). .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of PEI and plasmids, including Article Title: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells. Article Snippet: .. Transient transfection was performed in a 6-well plate using InvitrogenTM LipofectamineTM 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg of the PD-L1 expression plasmid, Article Title: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells Article Snippet: .. Transient transfection was performed in a 6-well plate using InvitrogenTM LipofectamineTM 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg of the PD-L1 expression plasmid, Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins. Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL-1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including Incubation:Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL −1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins. Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of PEI and plasmids including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins Article Snippet: HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL −1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including pEGFP-N1/PD-L1 (addgene Cat# 121478) and BFP-Rab5 (addgene Cat# 49147) in serum-free DMEM for 24 h. The transfected cells were further incubated with 0.5 μM CVR-EPLA for various times, fixed with a 4% PFA solution for 15 min, and finally imaged using a confocal laser scanning microscope (NIS Viewer 5.21 and NIS-Elements Viewer 5.21.00, Nikon, Japan). .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of PEI and plasmids, including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins. Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL-1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including Laser-Scanning Microscopy:Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL −1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins. Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of PEI and plasmids including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins Article Snippet: HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL −1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including pEGFP-N1/PD-L1 (addgene Cat# 121478) and BFP-Rab5 (addgene Cat# 49147) in serum-free DMEM for 24 h. The transfected cells were further incubated with 0.5 μM CVR-EPLA for various times, fixed with a 4% PFA solution for 15 min, and finally imaged using a confocal laser scanning microscope (NIS Viewer 5.21 and NIS-Elements Viewer 5.21.00, Nikon, Japan). .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of PEI and plasmids, including Article Title: Endosome-phagophore linking assemblies for the degradation of membrane/extracellular proteins. Article Snippet: .. HEK293T cells were transfected with the 3:1 (mass ratio) complexes of polyethylenimine (PEI, 1 mg mL-1 and pH 6.9–7.1, Kyfora Bio Cat# 23966) and plasmids including Expressing:Article Title: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells. Article Snippet: .. Transient transfection was performed in a 6-well plate using InvitrogenTM LipofectamineTM 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg of the PD-L1 expression plasmid, Article Title: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells Article Snippet: .. Transient transfection was performed in a 6-well plate using InvitrogenTM LipofectamineTM 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg of the PD-L1 expression plasmid, Plasmid Preparation:Article Title: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells. Article Snippet: .. Transient transfection was performed in a 6-well plate using InvitrogenTM LipofectamineTM 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg of the PD-L1 expression plasmid, Article Title: BRD4/nuclear PD-L1/RelB circuit is involved in the stemness of breast cancer cells Article Snippet: .. Transient transfection was performed in a 6-well plate using InvitrogenTM LipofectamineTM 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg of the PD-L1 expression plasmid, Article Title: FAT4 overexpression promotes antitumor immunity by regulating the β-catenin/STT3/PD-L1 axis in cervical cancer. Article Snippet: 7 d later, cell colonies were stained with Giemsa (G1015, Solarbio) and colonies of more than 50 cells were counted under the microscope. .. Article Title: Intracellular Localization of PD-L1 in Rab10-positive Open Tubular Endosome System of Cancer Cells Article Snippet: .. pSp-GFP-CD274(PD-L1)-NU was obtained from Dr. Christine Mayr, Sloan Kettering Institute (Addgene plasmid #121139), |